Fine Needle Aspirates
Collect sample directly into 30ml of CytoLyt solution. If specimen must be collected in an intravenous solution, use a balanced electrolyte solution.
Note: If possible, flush the needle and syringe with a sterile anticoagulant solution prior to aspirating the sample. Some anticoagulants may interfere with other cell processing techniques, so use caution if you plan to use the specimen for other testing.
Resuspension can be done on a vortexor or may be achieved by syringing the pellet back and forth with a plastic pipette.
If cell pellet is not free of blood, add 30ml of CytoLyt solution and repeat step 2.
(Fix, stain, and evaluate)